Cas 9 Antibody Biotin Conjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Cas 9 Antibody Biotin Conjugated, Rabbit, Polyclonal
• Artikelnummer: ROC-600-406-GK0
• Hersteller Artikelnummer: 600-406-GK0
• Alternativnummer: ROC-600-406-GK0
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA
• Spezies Reaktivität: Bacteria
• Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.
• Konjugation: Biotin
• Alternative Synonym: rabbit anti-Cas9 antibody biotin conjugation, biotin conjugated rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/mL by UV absorbance at 280 nm
• NCBI: 901176
• UniProt: Q99ZW2
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:25,000-1:30,000, IF Microscopy: 1µg/mL, WB: 1µg/mL
• Anwendungsbeschreibung: Cas9 Biotin Conjugated Antibody has been tested for use in ELISA and suitable for Immunofluorescence and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 prote

Bilder

Western Blot of Rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.

Immunofluorescence of Rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.