SARS-CoV-2 Nucleocapsid (N) Protein Antibody Biotin Conjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: SARS-CoV-2 Nucleocapsid (N) Protein Antibody Biotin Conjugated, Rabbit, Polyclonal
• Artikelnummer: ROC-600-406-MS4
• Hersteller Artikelnummer: 600-406-MS4
• Alternativnummer: ROC-600-406-MS4
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA
• Spezies Reaktivität: Virus
• Immunogen: Anti-SARS-CoV-2 Nucleocapsid (N) Protein Biotin Conjugated Antibody was produced by repeated immunizations with purified recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
• Konjugation: Biotin
• Alternative Synonym: 2019-nCoV, COVID-19, N-protein antibody, SARS CoV2 antibody, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2, rabbit anti-SARS CoV 2 Nucleocapsid Protein Antibody, sars-cov-2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml by UV absorbance at 280 nm
• NCBI: 43740575
• UniProt: P0DTC9
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: SARS
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:350,000-1:450,000, WB: User Optimized
• Anwendungsbeschreibung: Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody conjugated to biotin has been tested for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to SARS-CoV-2 Nucleo

Bilder

ELISA results of Rabbit Anti-SARS CoV-2 Nucleocapsid (N) Protein. Each well was coated in duplicate with 0.5 µg of SARS CoV-1 (N) Protein [Red Line], SARS CoV-2 (N) Protein [Green Line], MERS N [Blue Line]. The starting dilution of antibody was 0.5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS CoV-1 N IC50: 3ng/mL, SARS CoV-2 N IC50: 2ng/mL, MERS N IC50: 25mg/mL. Assay performed using Goat Anti-Rabbit IgG HRP conjugated (p/n 611-1302) and TMB substrate (p/n TMBE-1000).

Surface Plasmon Resonance (SPR) Data of Rabbit Anti-SARS-CoV-2 Nucleocapsid (N) Antibody. SARS-CoV-2 Nucleocapsid protein was immobilized on a carboxymethyldextran sensor chip. Ten (10) dilutions of Anti-SARS-CoV-2 Nucleocapsid (N) Antibody (colors) were flowed across the chip at 30 µL/min with 240 s contact time and then 720 s dissociation time. Fitted line (black) constants are: kon = 2.2 x 104 M-1 s-1 and koff = 3.3 x 10-4 s-1. The Equilibrium Dissociation constant (KD) of the antibody on several assays was 15 nM. (Average primary antibody KD = 1 µM).

ELISA Results of Rabbit Anti-SARS CoV-2 Nucleocapsid (N) Protein Antibody Biotin Conjugated. Each well was coated in duplicate with 10µg/mL of SARS CoV-2 N Protein [Blue Line]. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Streptavidin-Peroxidase (p/n S000-03) at 1:10,000, 3% Fish Gel/PBS (p/n MB-066), HRP Conjugate Stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).