SARS-CoV-2 Whole Spike Protein Antibody Biotin Conjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: SARS-CoV-2 Whole Spike Protein Antibody Biotin Conjugated, Rabbit, Polyclonal
• Artikelnummer: ROC-600-406-MS9
• Hersteller Artikelnummer: 600-406-MS9
• Alternativnummer: ROC-600-406-MS9
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA
• Spezies Reaktivität: Virus
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified SARS-CoV-2 S protein with His-tag, derived from the transfected human HEK293 cells.
• Konjugation: Biotin
• Alternative Synonym: 2019-nCoV, BAC conjugated SARS CoV-2 Spike Protein, COVID-19, S glycoprotein, SARS CoV2 antibody, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), Spike glycoprotein antibody, rabbit anti-SARS CoV 2 Spike Protein Antibody, sars-cov-2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 908947
• UniProt: P0DTC2
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: SARS
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:335,000 - 1:435,000, WB: User Optimized
• Anwendungsbeschreibung: Anti-SARS-CoV-2 Whole Spike protein antibody has been tested for use in ELISA and shows reactivity to SARS COV-2 Whole Spike. Specific conditions for reactivity should be optimized by the end user.

Bilder

ELISA results of Rabbit Anti-SARS CoV-2 Whole Spike Protein. Each well was coated in duplicate with 0.5µg of rec SARS Cov-2 whole spike protein [RED line], rec SARS Cov-2 spike (S2), SHFc [GREEN line], rec MERS Spike S1 protein [BLUE line], and rec SARS Cov-2 N protein [PURPLE line]. The working dilution for SARS-Cov-2 whole spike is 1:168,000 and SARS Cov-2 spike S2, SHFc is 1:66,400. The starting dilution of antibody was 0.55µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS-Cov-2 whole spike EC50: 6ng/mL and SARS Cov-2 spike S2 EC50: 15ng/mL.

Sandwich ELISA Report for the detection of SARS-CoV-2 Spike Protein. Capture Antibody: Anti-SARS-CoV-2 Whole Spike Protein (RABBIT) Antibody (p/n 600-401-MS9) [4 µg/mL]. Detection Antibody: Anti-SARS-CoV-2 Whole Spike Protein (RABBIT) Antibody Biotin Conjugated (p/n 600-406-MS9) [0.6 µg/mL]. Reporter: Streptavidin Peroxidase Conjugated (p/n S000-03) [1:10,000]. SARS-CoV-2 was serial diluted from 6000 pg/mL to 5.8 pg/mL. Analysis confirms sensitivity to 11.7 pg/mL.

ELISA Results of Rabbit Anti-SARS CoV-2 Whole Spike Protein Biotin Conjugated. Each well was coated in duplicate with 10µg/mL of SARS CoV-2 Whole Spike Protein [Red Line]. The working dilution is 1:384,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 1% Fish Gel/PBS (p/n MB-066), HRP Stabilizer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03) at 1:10,000, and TMB substrate (p/n TMBE-1000).