Anti-Tryptophan Hydroxylase Antibody was produced in sheep by repeated immunizations with recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
Anti-Tryptophan Hydroxylase (Sheep) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 55 kDa in size corresponding to tryptophan hydroxylase prot
Western Blot of Sheep anti-Tryptophan Hydroxylase antibody. Lane 1: human dorsal Raphe nucleus. Lane 2: None. Load: 10 µg per lane. Primary antibody: Tryptophan Hydroxylase antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) sheep secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa for Tryptophan Hydroxylase. Other band(s): none.
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