Tryptophan Hydroxylase Antibody, Sheep, Polyclonal

Artikelnummer: ROC-600-601-C96
Artikelname: Tryptophan Hydroxylase Antibody, Sheep, Polyclonal
Artikelnummer: ROC-600-601-C96
Hersteller Artikelnummer: 600-601-C96
Alternativnummer: ROC-600-601-C96
Hersteller: Rockland Immunochemicals
Wirt: Sheep
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Rat
Immunogen: Anti-Tryptophan Hydroxylase Antibody was produced in sheep by repeated immunizations with recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
Konjugation: Unconjugated
Alternative Synonym: Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1
Klonalität: Polyclonal
NCBI: 100101558
UniProt: P17290
Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Formulierung: Liquid
Target-Kategorie: Rabbit
Antibody Type: Primary Antibody
Application Verdünnung: IHC: 1:1000, WB: 1:1000
Anwendungsbeschreibung: Anti-Tryptophan Hydroxylase (Sheep) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 55 kDa in size corresponding to tryptophan hydroxylase prot
Western Blot of Sheep anti-Tryptophan Hydroxylase antibody. Lane 1: human dorsal Raphe nucleus. Lane 2: None. Load: 10 µg per lane. Primary antibody: Tryptophan Hydroxylase antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) sheep secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa for Tryptophan Hydroxylase. Other band(s): none.