Anti-Dopamine beta Hydroxylase Antibody was produced in sheep by repeated immunizations with synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH.
Anti-Dopamine beta-Hydroxylase Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 75 kDa in size corresponding to DBH protein in the appropriate
Western Blot of Anti-Dopamine ß-Hydroxylase (Sheep) Antibody. Lane 1: human adrenal medulla. Lane 2: none. Load: 10 µg per lane. Primary antibody: Dopamine ß-Hydroxylase antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) sheep secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~75kDa/~75kDa for DBH protein. Other band(s): none.
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