Rabbit IgG anti-Bovine IgG (Fc)-HRPO, MinX none

Artikelnummer: ROC-601-4303
Artikelname: Rabbit IgG anti-Bovine IgG (Fc)-HRPO, MinX none
Artikelnummer: ROC-601-4303
Hersteller Artikelnummer: 601-4303
Alternativnummer: ROC-601-4303
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Bovine
Immunogen: Bovine IgG F(c) fragment
Konjugation: HRPO
Alternative Synonym: rabbit anti-Bovine IgG F(c) peroxidase Conjugated Antibody, rabbit anti-Bovine IgG Fc HRP Conjugated Antibody
Format: IgG
Spezifität: IgG (Fc)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 1.5 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Bovine
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Anwendungsbeschreibung: Anti-Bovine IgG F(c) Peroxidase Conjugated Antibody has been tested by ELISA. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a s
ELISA Results of Rabbit Anti-Bovine IgG Fc Antibody Peroxidase Conjugated tested against purified Bovine IgG Fc HRP. Each well was coated in duplicate with 1.0 µg of Bovine IgG F(c) (p/n 001-0103). The working dilution is 1:15,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.