Rabbit IgG anti-Bovine IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Produktübersicht

• Artikelname: Rabbit IgG anti-Bovine IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Artikelnummer: ROC-601-4502
• Hersteller Artikelnummer: 601-4502
• Alternativnummer: ROC-601-4502
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Bovine
• Immunogen: Bovine IgG whole molecule
• Konjugation: Alk. Phos.
• Alternative Synonym: rabbit anti-Bovine IgG Alkaline Phosphatase Conjugated Antibody, rabbit anti-Bovine IgG Alk phos Conjugated Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Bovine
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:1,000 - 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: Anti-Bovine IgG Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-

Bilder

ELISA Results of Rabbit Anti-Bovine IgG Antibody Alkaline Phosphatase Conjugated tested against purified Bovine IgG Alk Phos. Each well was coated in duplicate with 1.0 µg of Bovine IgG (p/n 001-0102). The working dilution is 1:60,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).