Chicken IgM (mu chain) Antibody Rhodamine Conjugated, TRITC, Goat, Polyclonal

Artikelnummer: ROC-603-100-007
Artikelname: Chicken IgM (mu chain) Antibody Rhodamine Conjugated, TRITC, Goat, Polyclonal
Artikelnummer: ROC-603-100-007
Hersteller Artikelnummer: 603-100-007
Alternativnummer: ROC-603-100-007
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Spezies Reaktivität: Gallus
Immunogen: Chicken IgM whole molecule
Konjugation: TRITC
Alternative Synonym: goat anti-Chicken IgM (mu chain) Antibody Rhodamine Conjugation, goat anti-Chicken IgM mu chain TRITC Conjugated Antibody
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Chicken
Antibody Type: Secondary Antibody
Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
Anwendungsbeschreibung: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor
Western Blot of Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated (p/n 603-103-007). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG F(ab) Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated (p/n 603-103-007) 1:30,000 for 60 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.