Chicken IgG (H&L) Antibody Texas Red(TM) Conjugated, Goat, Polyclonal

Artikelnummer: ROC-603-109-002
Artikelname: Chicken IgG (H&L) Antibody Texas Red(TM) Conjugated, Goat, Polyclonal
Artikelnummer: ROC-603-109-002
Hersteller Artikelnummer: 603-109-002
Alternativnummer: ROC-603-109-002
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Gallus
Immunogen: Chicken IgG whole molecule
Konjugation: Texas Red
Alternative Synonym: goat anti-Chicken IgG Texas Red(TM) Conjugated Antibody, goat anti-Chicken IgY Antibody Texas Red(TM) Conjugation, Chicken Secondary Antibody
Klonalität: Polyclonal
Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Chicken
Antibody Type: Secondary Antibody
Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
Anwendungsbeschreibung: Anti-Chicken IgG Texas Red Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, includi
Western Blot of Unconjugated Anti-Chicken IgG/IgY (H&L) (GOAT) Antibody (p/n 603-101-002). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG/IgY (H&L) (GOAT) Antibody (p/n 603-101-002) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.