Chicken IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed, Cy2, Goat, Polyclonal

Produktübersicht

• Artikelname: Chicken IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed, Cy2, Goat, Polyclonal
• Artikelnummer: ROC-603-111-126
• Hersteller Artikelnummer: 603-111-126
• Alternativnummer: ROC-603-111-126
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Spezies Reaktivität: Gallus
• Immunogen: Chicken IgG whole molecule
• Konjugation: Cy2
• Alternative Synonym: goat anti-Chicken IgG Antibody CY2 Conjugation, goat anti-Chicken IgG CY2 Conjugated Antibody, goat anti-Chicken IgY CY2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Chicken
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: User Optimized, FLISA: 1:10,000 - 1:50,000, Flow Cytometry: User Optimized, IHC: User Optimized, IF Microscopy: 1:1,000 - 1:5,000, WB: User Optimized
• Anwendungsbeschreibung: Cy2 Conjugated secondary antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing vario

Bilder

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-101-126). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG/IgY whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). Lane 5: Chicken Serum (p/n D302-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-101-126) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.