Chicken IgG (H&L) Antibody Rhodamine Conjugated, TRITC, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Chicken IgG (H&L) Antibody Rhodamine Conjugated, TRITC, Rabbit, Polyclonal
• Artikelnummer: ROC-603-4002
• Hersteller Artikelnummer: 603-4002
• Alternativnummer: ROC-603-4002
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Spezies Reaktivität: Gallus
• Immunogen: Chicken IgG whole molecule
• Konjugation: TRITC
• Alternative Synonym: rabbit anti-Chicken IgG Antibody Rhodamine Conjugation, rabbit anti-Chicken IgG TRITC Conjugated Antibody, rabbit anti-Chicken IgY Antibody Rhodamine Conjugation, rabbit anti-Chicken IgY TRITC Conjugated Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.5 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Chicken
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor

Bilder

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.