Rabbit IgG anti-Chicken IgG (H+L)-FITC, MinX none

Produktübersicht

• Artikelname: Rabbit IgG anti-Chicken IgG (H+L)-FITC, MinX none
• Artikelnummer: ROC-603-4202-0500
• Hersteller Artikelnummer: 603-4202-0500
• Alternativnummer: ROC-603-4202-0500
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FLISA,FACS,IF
• Spezies Reaktivität: Gallus
• Immunogen: Chicken IgG whole molecule
• Konjugation: FITC
• Alternative Synonym: rabbit anti-Chicken IgG Antibody Fluorescein Conjugation, rabbit anti-Chicken IgG FITC Conjugated Antibody, rabbit anti-Chicken IgY Antibody Fluorescein Conjugation, rabbit anti-Chicken IgY FITC Conjugated Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Chicken
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: Anti-Chicken IgG Fluorescein Antibody has been tested by dot blot and western blot. This product is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background le

Bilder

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.