Chicken IgG F(ab)2 Antibody Texas Red(TM) Conjugated, Rabbit, Polyclonal

Artikelnummer: ROC-603-4904
Artikelname: Chicken IgG F(ab)2 Antibody Texas Red(TM) Conjugated, Rabbit, Polyclonal
Artikelnummer: ROC-603-4904
Hersteller Artikelnummer: 603-4904
Alternativnummer: ROC-603-4904
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Spezies Reaktivität: Gallus
Immunogen: Chicken IgG F(ab)2 fragment
Konjugation: Texas Red
Alternative Synonym: rabbit anti Chicken IgG F(ab)2 Antibody Texas Red(TM) conjugate, rabbit anti Chicken IgG Fab2 Antibody Texas Red(TM) Conjugate, rabbit anti-Chicken IgY F(ab)2 Antibody Texas Red(TM)
Klonalität: Polyclonal
Konzentration: 1.5 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Chicken
Antibody Type: Secondary Antibody
Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
Anwendungsbeschreibung: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor
Western Blot of Unconjugated Anti-Chicken IgG F(ab)2 (RABBIT) Antibody (p/n 603-4104). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG F(ab) Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(ab)2 (RABBIT) Antibody (p/n 603-4104) 1:5,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.