Goat IgG anti-Dog IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Produktübersicht

• Artikelname: Goat IgG anti-Dog IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Artikelnummer: ROC-604-1502
• Hersteller Artikelnummer: 604-1502
• Alternativnummer: ROC-604-1502
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Canine
• Immunogen: Dog IgG whole molecule
• Konjugation: Alk. Phos.
• Alternative Synonym: Goat anti-Dog IgG Antibody Alkaline Phosphatase Conjugation, Goat anti-Dog IgG Alk Phos Conjugated Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Dog
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: Anti-Dog IgG Alkaline Phosphatase Conjugated has been tested by ELISA. This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature.

Bilder

ELISA Results of Goat Anti-Dog IgG Antibody Alkaline Phosphatase Conjugated tested against purified Dog IgG Alk Phos. Each well was coated in duplicate with 1.0 µg of Dog IgG (p/n 004-0102). The working dilution is 1:8000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase Substrate ELISA Buffer (p/n NPP-10) and NPP Working Buffer (p/n NPP-B500).