Goat IgG anti-Dog IgG (Fc)-Biotin, MinX none

Produktübersicht

• Artikelname: Goat IgG anti-Dog IgG (Fc)-Biotin, MinX none
• Artikelnummer: ROC-604-1603
• Hersteller Artikelnummer: 604-1603
• Alternativnummer: ROC-604-1603
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Canine
• Immunogen: Dog IgG F(c) fragment
• Konjugation: Biotin
• Alternative Synonym: goat anti-Dog IgG F(c) Antibody Biotin conjugation, goat anti-Dog IgG Fc fragment biotin conjugated Antibody, Dog Secondary Antibody
• Format: IgG
• Spezifität: IgG (Fc)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Dog
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-DOG IgG F(c) Biotin Conjugated Antibody has been tested by ELISA and is assayed against 1.0 ug of Dog IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])

Bilder

ELISA Results of Goat Anti-Dog IgG F(c) Antibody Biotin Conjugated tested against purified Dog IgG F(c). Each well was coated in duplicate with 1.0 µg of Dog IgG F(c) (p/n 004-0103). The working dilution is 1:88,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP stabilizer (p/n MB-076), Streptavidin HRP conjugated (p/n S000-03) and TMB substrate (p/n TMBE-1000).