Donkey IgG anti-Goat IgG (H+L)-unconj., MinX Ho,Ms, Polyclonal , Unconjugated

Produktübersicht

• Artikelname: Donkey IgG anti-Goat IgG (H+L)-unconj., MinX Ho,Ms, Polyclonal , Unconjugated
• Artikelnummer: ROC-605-701-125
• Hersteller Artikelnummer: 605-701-125
• Alternativnummer: ROC-605-701-125
• Hersteller: Rockland Immunochemicals
• Wirt: Donkey
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Goat
• Immunogen: Goat IgG whole molecule
• Konjugation: Unconjugated
• Alternative Synonym: donkey anti-goat IgG antibody pre-adsorbed
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): Equine,Mouse

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.25 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Goat
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:150,000 - 1:250,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Goat IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Bilder

ELISA Results of Donkey Anti-Goat IgG Antibody (Min X Ch GP Ham Hs Ms Rb Rt Serum proteins) tested against purified Goat IgG MX7. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n 005-0102) as well as Chicken IgG (p/n 003-0102), Guinea Pig IgG (p/n 006-0102), Hamster IgG (p/n 007-0102), Horse IgG (p/n 008-0102), Mouse IgG (p/n 010-0102), Rabbit IgG (p/n 011-0102), and Rat IgG (p/n 012-0102). The working dilution is 1:56,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Goat Anti-Donkey IgG Mx7 HRP conjugated and TMB substrate (p/n TMBE-1000).