Donkey IgG anti-Goat IgG (H+L)-Biotin, MinX none

Produktübersicht

• Artikelname: Donkey IgG anti-Goat IgG (H+L)-Biotin, MinX none
• Artikelnummer: ROC-605-706-002
• Hersteller Artikelnummer: 605-706-002
• Alternativnummer: ROC-605-706-002
• Hersteller: Rockland Immunochemicals
• Wirt: Donkey
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Goat
• Immunogen: Anti-Goat IgG (H&L) was produced by repeated immunization with goat IgG whole molecule in donkey.
• Konjugation: Biotin
• Alternative Synonym: donkey anti-Goat IgG Antibody biotin Conjugation, donkey anti-Goat IgG biotin Conjugated Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Goat
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:200,000 - 1:250,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Goat IgG Biotin Conjugated Antibody has been tested by ELISA and Dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring

Bilder

ELISA results of purified Donkey anti-Goat IgG antibody Biotin conjugated tested against purified Goat IgG. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n 005-0102-0010). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, and TMB substrate p/n TMBE-1000.