Goat IgG anti-Horse IgG (H+L)-HRPO, MinX none

Artikelnummer: ROC-608-1302
Artikelname: Goat IgG anti-Horse IgG (H+L)-HRPO, MinX none
Artikelnummer: ROC-608-1302
Hersteller Artikelnummer: 608-1302
Alternativnummer: ROC-608-1302
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Equine
Immunogen: Horse IgG whole molecule
Konjugation: HRPO
Alternative Synonym: goat Anti-Horse IgG peroxidase Conjugated Antibody, goat Anti-Horse IgG Antibody HRP Conjugation
Format: IgG
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Horse
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:20,000 - 1:60,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000
Anwendungsbeschreibung: Anti-Horse IgG Peroxidase Antibody has been tested by ELISA and is designed for western blotting and Immunohistochemistry.
ELISA Results of Goat Anti-Horse IgG Antibody Peroxidase Conjugated tested against purified Horse IgG. Each well was coated in duplicate with 1.0 µg of Horse IgG (p/n 008-0102). The working dilution is 1:400,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Horse IgG HRP conjugated and TMB substrate (p/n TMBE-1000).