Goat IgG anti-Human IgG (Fc)-HRPO, MinX Ms, Polyclonal

Produktübersicht

• Artikelname: Goat IgG anti-Human IgG (Fc)-HRPO, MinX Ms, Polyclonal
• Artikelnummer: ROC-609-103-017
• Hersteller Artikelnummer: 609-103-017
• Alternativnummer: ROC-609-103-017
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Human
• Immunogen: Human IgG F(c) fragment
• Konjugation: HRPO
• Alternative Synonym: goat anti-Human IgG F(c) peroxidase Conjugated Antibody, goat anti-Human IgG F(c) fragment antibody HRP conjugation
• Format: IgG
• Spezifität: IgG (Fc)
• Minimale Kreuzreaktivität (MinX): Mouse

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Human
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Human IgG F(c) Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot co

Bilder

ELISA Results of purified Goat Anti-Human IgG F(c) Antibody Peroxidase Conjugated tested against purified Human IgG F(c) HRP. Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n 009-0103) [Blue Line] and Human IgG (p/n 009-0102) [Purple Line]. The working dilution is 1:33,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).