Goat IgG anti-Human IgG (H+L)-Alk. Phos., MinX Bo,Ck,Go,Gp,Hm,Ho,Ms,Rb,Rt,Sh, ALP, Polyclonal , AP

Produktübersicht

• Artikelname: Goat IgG anti-Human IgG (H+L)-Alk. Phos., MinX Bo,Ck,Go,Gp,Hm,Ho,Ms,Rb,Rt,Sh, ALP, Polyclonal , AP
• Artikelnummer: ROC-609-105-123
• Hersteller Artikelnummer: 609-105-123
• Alternativnummer: ROC-609-105-123
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Human
• Immunogen: Human IgG whole molecule
• Konjugation: Alk. Phos.
• Alternative Synonym: goat anti-Human IgG Alkaline Phosphatase Conjugation, goat anti-Human IgG ALP conjugate, goat anti-human IgG Alk Phos conjugated antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Mouse,Rabbit,Rat,Sheep

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000 - 1:30,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: Anti-Human IgG Alkaline Phosphatase conjugate has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requi

Bilder

ELISA Results of Goat Anti-Human IgG Antibody Alkaline Phosphatase Conjugated (Min X Bv Ch Gt GP Ham Hs Ms Rb Rt Sh Serum Proteins) tested against purified Human IgG Alk Phos. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n 009-0102). The working dilution is 1:19,600. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Alkaline Phosphatase Substrate Buffer (p/n NPP-10) and NPP Working Buffer (p/n NPP-B500).