Goat IgG anti-Human IgG (H+L)-Alk. Phos., MinX Ms, ALP, Polyclonal , AP

Artikelnummer: ROC-609-1516
Artikelname: Goat IgG anti-Human IgG (H+L)-Alk. Phos., MinX Ms, ALP, Polyclonal , AP
Artikelnummer: ROC-609-1516
Hersteller Artikelnummer: 609-1516
Alternativnummer: ROC-609-1516
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Human
Immunogen: Human IgG whole molecule
Konjugation: Alk. Phos.
Alternative Synonym: goat anti-Human IgG Alkaline Phosphatase Conjugation, goat anti-Human IgG ALP conjugate, goat anti-human IgG Alk Phos conjugated antibody
Format: IgG
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): Mouse
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Anwendungsbeschreibung: Anti-Human IgG (H&L) Alkaline Phosphatase has been tested by dot blot and ELISA and is assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature. A w
Dot Blot of Goat anti-Human IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed. Antigen: Human IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Human IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed at 1:1,000 for 1 HR at RT. Block: MB-070 for 1 HR at RT.