Anti-Human IgG (H&L) Alkaline Phosphatase has been tested by dot blot and ELISA and is assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature. A w
Dot Blot of Goat anti-Human IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed. Antigen: Human IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Human IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed at 1:1,000 for 1 HR at RT. Block: MB-070 for 1 HR at RT.
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