Anti-Parkin pS378 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser378.
Anti-Parkin pS378 Antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 52 kDa in size corresponding to parkin protein phosphorylated at Ser378 in the approp
Western Blot of Rabbit anti-Parkin pS378 antibody. Lane 1: HEK293 cells transfected with Parkin WT (Phospho). Lane 2: HEK293 cells transfected with Parkin S378 mutant (non-phospho). Load: 10 µg per lane. Primary antibody: Parkin pS378 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 52 kDa for Parkin pS378. Other band(s): none.
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