Sheep IgG anti-Human IgG (H+L)-unconj., MinX none

Produktübersicht

• Artikelname: Sheep IgG anti-Human IgG (H+L)-unconj., MinX none
• Artikelnummer: ROC-609-6102
• Hersteller Artikelnummer: 609-6102
• Alternativnummer: ROC-609-6102
• Hersteller: Rockland Immunochemicals
• Wirt: Sheep
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Human
• Immunogen: Human IgG whole molecule
• Konjugation: Unconjugated
• Alternative Synonym: Sheep Anti Human IgG Antibody, Sheep Anti-Human IgG Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.1 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:30,000 - 1:60,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:20,000
• Anwendungsbeschreibung: Anti-Human IgG antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Bilder

ELISA Results of Sheep Anti-Human IgG Antibody tested against purified Human IgG. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n 009-0102). The working dilution is 1:60000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Donkey Anti-Sheep IgG HRP conjugated (p/n 613-703-168) and TMB substrate (p/n TMBE-1000).