Goat IgG anti-Mouse IgG (F(ab)2)-HRPO, MinX none

Produktübersicht

• Artikelname: Goat IgG anti-Mouse IgG (F(ab)2)-HRPO, MinX none
• Artikelnummer: ROC-610-1304
• Hersteller Artikelnummer: 610-1304
• Alternativnummer: ROC-610-1304
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgG F(ab)2 fragment
• Konjugation: HRPO
• Alternative Synonym: Goat Anti-Mouse IgG F(ab)2 peroxidase Conjugated Antibody, Goat Anti-Mouse IgG Fab2 peroxidase Conjugated Antibody, Goat Anti-Mouse IgG Fab2 Fragment Antibody HRP Conjugation
• Format: IgG
• Spezifität: IgG (F(ab')2)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: Anti-MOUSE IgG F(ab)2 Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal dete

Bilder

ELISA Results of Goat Anti-Mouse IgG F(ab)2 Antibody Peroxidase Conjugated tested against purified Mouse IgG F(ab)2. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 010-0102) [Red Line], Mouse IgG F(ab)2 (p/n 010-0104) [Green Line]. The working dilution is 1:38,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).