Rabbit IgG anti-Mouse IgG (Fc)-unconj., MinX none

Produktübersicht

• Artikelname: Rabbit IgG anti-Mouse IgG (Fc)-unconj., MinX none
• Artikelnummer: ROC-610-4103
• Hersteller Artikelnummer: 610-4103
• Alternativnummer: ROC-610-4103
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Mouse
• Immunogen: Anti-Mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in rabbit.
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-Mouse IgG F(c) Antibody, Rb-a-Mouse F(c), Mouse F(c) Antibody in rabbit, Mouse F(c) Secondary Antibody
• Format: IgG
• Spezifität: IgG (Fc)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.1 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:100,000 - 1:250,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Mouse IgG F(c) Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Bilder

ELISA of Rabbit Anti-Mouse IgG F(c) Antibody tested against purified Mouse IgG F(c). Each well was coated in duplicate with 1.0 µg of Mouse IgG F(c) (p/n 010-0103) [Red Line], Mouse IgG Fab (p/n 010-0105) [Green line], Mouse IgG F(ab)2 (p/n 010-0104) [Blue Line], and Mouse IgG (p/n 010-0102) [Purple Line]. The working dilution of Mouse IgG F(c) is 1:53000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Goat Anti-Rabbit IgG HRP conjugated (p/n 611-103-122), and TMB substrate (p/n TMBE-1000).