Rabbit IgG anti-Mouse IgM (µ)-unconj., MinX none

Produktübersicht

• Artikelname: Rabbit IgG anti-Mouse IgM (µ)-unconj., MinX none
• Artikelnummer: ROC-610-4107
• Hersteller Artikelnummer: 610-4107
• Alternativnummer: ROC-610-4107
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgM whole molecule
• Konjugation: Unconjugated
• Alternative Synonym: Rabbit Anti-Mouse IgM (mu chain) Antibody, Rabbit Anti-Mouse IgM mu chain Antibody
• Format: IgG
• Spezifität: IgM (µ)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.2 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Mouse IgM mu heavy chain antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should b

Bilder

ELISA Results of Rabbit Anti-Mouse IgM Antibody tested against purified Mouse IgM. Each well was coated in duplicate with 10 µg of Mouse IgM (p/n 010-0107). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066), Goat Anti-Rabbit IgG mx10 HRP conjugated (p/n 611-103-122), and TMB substrate (p/n TMBE-1000).