Anti-Mouse IgG (H&L) Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA results of purified Rabbit anti-Mouse IgG Antibody (min x Human Serum Proteins) tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 010-0102) [red line] and Human IgG (p/n 009-0102) [blue line]. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-103-122) and TMB ELISA Substrate (p/n TMBE-1000).
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