Rabbit IgG anti-Mouse IgG2b (Fc)-unconj., MinX none

Produktübersicht

• Artikelname: Rabbit IgG anti-Mouse IgG2b (Fc)-unconj., MinX none
• Artikelnummer: ROC-610-4142
• Hersteller Artikelnummer: 610-4142
• Alternativnummer: ROC-610-4142
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgG2b heavy chain
• Konjugation: Unconjugated
• Alternative Synonym: Rabbit Anti-Mouse IgG2b (Gamma 2b chain) Antibody, Rabbit Anti Mouse IgG2b Antibody
• Format: IgG
• Spezifität: IgG2b (Fc)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Mouse IgG2b antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Bilder

ELISA results of purified Rabbit anti-Mouse IgG2b (Gamma 2B Chain) antibody tested against purified Mouse IgG2b. Each well was coated in duplicate with 1.0 µg of Mouse IgG2b (p/n 010-0142). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-103-122) and TMB substrate p/n TMBE-1000.