Anti-Mouse IgG2b peroxidase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic a
Induction of HBV core-specific antibodies in B6 and TLR7-/- mice. B6 and TLR-7-/- (n=4/4) were immunized with recombinant HEK-293-derived stC149tat particles. Serum samples were obtained and analysed as described in the M&M section. Mean specific antibody titers in sera SD (a) and the calculated IgG1/IgG2a ratios SD (b) of a representative experiment (out of two performed experiments) are shown. The statistical significance of differences in IgG and IgG2b antibody titers between stC149tat immune B6 and TLR7-/- mice was determined by the unpaired Students t-test. P values of <0.05 (*) and <0.001 (***) were considered statistically significant. Figure provided by CiteAb. Source: Sci Rep, PMID: 30279478.
Expression and characterization of a mutant HBV-stCAAA and HBV-stCAAAY132A antigen. (a) Samples of purified stCAAA and stCAAAY132A antigens were processed for SDS-PAGE and subsequent Coomassie Blue staining of the gel. The positions of the respective Core antigens and the C1QBP co-precipitating with stCAAAY132A are indicated. The molecular weight marker in kDa is shown. (b) Same amounts of stCAAA and the non-particulate stCAAAY132A (2µg, calculated for same amounts of monomers determined by SDS-PAGE) were subjected to native agarose gels stained with ethidium bromide (EB) and subsequent with Coomassie Blue (CB). The original gel used to generate this cropped figure is shown in Supplementary Fig.S9. (c) B6 mice were immunized with recombinant HEK-293 derived stCAAA and stCAAAY132A antigens (n=5/6). Serum samples were obtained 21 days post injection and analyzed for Core-specific IgG, IgG1 and IgG2b antibody titers by end-point dilution ELISA using bacterial rHBV-C149 as detection antigen. Mean specific antibody titers in sera SD of a representative experiment (out of two performed experiments) (d) and the calculated IgG1/IgG2a ratios SD are shown. (c and d) Statistically significant differences between the group 1 and group 2 were determined using the unpaired students t-test. P values of <0.01 (**) and p<0.001 (***) were considered statistically significant. Figure provided by CiteAb. Source: Sci Rep, PMID: 30279478.
Induction of HBV core-specific antibodies in mice. (a) B6 mice were immunized with recombinant HEK-293-derived stC149tat or stC149 (n=4/5). Three weeks post injection serum samples were obtained by tail bleeding and HBV core-specific IgG, IgG1 and IgG2b serum antibody titers were determined by end-point dilution ELISA using bacterial rHBV-C149 particles as detection antigen. Mean specific antibody titers in sera SD (a) and the calculated IgG1/IgG2a ratios SD (b) of a representative experiment (out of two performed experiments) are shown. The statistical significance of differences in IgG, IgG1 and IgG2b antibody titers between stC149tat- and stC149 immune B6 mice were determined by the unpaired Students t-test. (c) B6 mice were immunized with recombinant stC149tat or stC149 proteins. Ten days post immunization spleen cells were stimulated ex vivo for 2 days with the HBV-Core-specific I-Ab-binding C128-140 peptide. The specific IFN-gamma release into the cell culture supernatants was determined by ELISA. The statistical significance of differences in IFN-gamma levels between stC149- and stC149tat-immune mice (groups 2 and 3) were determined by the unpaired Students t-test. (a-c) P values of <0.05 (*) and <0.01 (**) were considered statistically significant. Figure provided by CiteAb. Source: Sci Rep, PMID: 30279478.
ELISA Results of Rabbit Anti-Mouse IgG2b Antibody Peroxidase Conjugation tested against purified Mouse IgG2b HRP. Each well was coated in duplicate with 1.0 µg of Mouse IgG2b (p/n 010-0142). The working dilution is 1:82,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).
Characterization
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