Rabbit IgG anti-Mouse IgG3 (Fc)-HRPO, MinX none

Produktübersicht

• Artikelname: Rabbit IgG anti-Mouse IgG3 (Fc)-HRPO, MinX none
• Artikelnummer: ROC-610-4343
• Hersteller Artikelnummer: 610-4343
• Alternativnummer: ROC-610-4343
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Mouse
• Immunogen: Anti-Mouse IgG3 heavy chain was produced by repeated immunization with Mouse IgG3 heavy chain in rabbit.
• Konjugation: HRPO
• Alternative Synonym: Rabbit Anti-Mouse IgG3 (gamma 3 chain) Antibody peroxidase Conjugated, Rabbit Anti-Mouse IgG3 Antibody HRP Conjugation
• Format: IgG
• Spezifität: IgG3 (Fc)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:75,000 - 1:125,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: Mouse IgG3 heavy chain Peroxidase Conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and spe

Bilder

ELISA Results of Rabbit Anti-Mouse IgG3 Antibody Peroxidase Conjugated tested against purified Mouse IgG3 HRP. Each well was coated in duplicate with 1.0 µg of Mouse IgG3 (p/n 010-0143). The working dilution is 1:41600. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).