Rabbit IgG anti-Mouse IgM (µ)-Biotin, MinX none

Produktübersicht

• Artikelname: Rabbit IgG anti-Mouse IgM (µ)-Biotin, MinX none
• Artikelnummer: ROC-610-4607
• Hersteller Artikelnummer: 610-4607
• Alternativnummer: ROC-610-4607
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgM whole molecule
• Konjugation: Biotin
• Alternative Synonym: rabbit Anti-Mouse IgM (mu chain) Antibody Biotin Conjugated, rabbit Anti Mouse IgM mu chain Biotin Conjugated Antibody
• Format: IgG
• Spezifität: IgM (µ)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.5 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Mouse IgM Biotin Conjugated Antibody has been tested by ELISA and has been assayed against 1.0 ug of Mouse IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic ac

Bilder

ELISA Results of Rabbit Anti-Mouse IgM Antibody Biotin Conjugated tested against purified Mouse IgM Biotin. Each well was coated in duplicate with 1.0 µg of Mouse IgM (p/n 010-0107). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).