Rabbit IgG Fc Antibody Rhodamine Conjugated, TRITC, Goat, Polyclonal

Produktübersicht

• Artikelname: Rabbit IgG Fc Antibody Rhodamine Conjugated, TRITC, Goat, Polyclonal
• Artikelnummer: ROC-611-1003
• Hersteller Artikelnummer: 611-1003
• Alternativnummer: ROC-611-1003
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Spezies Reaktivität: Rabbit
• Immunogen: Rabbit IgG F(c) fragment
• Konjugation: TRITC
• Alternative Synonym: Goat anti-Rabbit IgG F(c) Antibody Rhodamine Conjugation, Goat anti-Rabbit IgG Fc fragment Antibody Rhodamine Conjugation, Goat anti-Rabbit IgG F(c) Rhodamine Conjugated Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rabbit
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor

Bilder

(a) Channels coating: remnant fluorescence due to nonspecific binding of anti-rabbit IgG labeled with B-rhodamine (p/n 611-1003) [AbII] at a concentration of 100µg/ml in uncoated and coated microchannels with silane-PEG. (b) Fluorescence obtained from the complete immunoassay at a flow rate of 5µl/h and at a anti-biotin rabbit IgG (p/n 100-4198) [AbI] concentration of 50pg/ml is compared to the fluorescence obtained from the immunoassay performed without applying the magnetic field (column 1), without adding anti-biotin rabbit IgG (p/n 100-4198) [AbI] (column 2), without adding ), anti-rabbit IgG labeled with B-rhodamine (p/n 611-1003) [AbII] (column 3), and, finally, the efficacy of the microparticles coating was tested by performing the immunoassay without anti-biotin rabbit IgG (p/n 100-4198) [AbI] and with noncoated microparticles (column 5). The level of fluorescence of the two first columns in b results from nonspecific interactions of anti-rabbit IgG labeled with B-rhodamine (p/n 611-1003) [AbII] with the microparticles. Error bars are standard deviation. FIG. 5. PMID: 32038740.