Rabbit IgG (H&L) Antibody Texas Red(TM) Conjugated Pre-Adsorbed, Goat, Polyclonal

Artikelnummer: ROC-611-109-122
Artikelname: Rabbit IgG (H&L) Antibody Texas Red(TM) Conjugated Pre-Adsorbed, Goat, Polyclonal
Artikelnummer: ROC-611-109-122
Hersteller Artikelnummer: 611-109-122
Alternativnummer: ROC-611-109-122
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: DOT
Spezies Reaktivität: Rabbit
Immunogen: Rabbit IgG whole molecule
Konjugation: Texas Red
Alternative Synonym: Goat Anti-Rabbit IgG Antibody Texas Red(TM) Conjugated, Goat Anti Rabbit IgG Texas Red(TM) Conjugated Antibody
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Rabbit
Antibody Type: Secondary Antibody
Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
Anwendungsbeschreibung: Anti-Rabbit IgG Antibody Texas Red(TM) has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including m
Western Blot of Unconjugated Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.