Goat IgG anti-Rabbit IgG (Fc)-FITC, MinX Hu, Polyclonal

Produktübersicht

• Artikelname: Goat IgG anti-Rabbit IgG (Fc)-FITC, MinX Hu, Polyclonal
• Artikelnummer: ROC-611-1223
• Hersteller Artikelnummer: 611-1223
• Alternativnummer: ROC-611-1223
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: FLISA,FACS,IF
• Spezies Reaktivität: Rabbit
• Immunogen: Rabbit IgG F(c) fragment
• Konjugation: FITC
• Alternative Synonym: Goat anti-Rabbit IgG F(c) Antibody fluorescein Conjugation, Goat anti-Rabbit IgG Fc fragment Antibody fluorescein Conjugation, Goat anti-Rabbit IgG F(c) FITC Conjugated Antibody
• Format: IgG
• Spezifität: IgG (Fc)
• Minimale Kreuzreaktivität (MinX): Human

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rabbit
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor

Bilder

Western Blot of Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n 611-1103). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n 611-1103) 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.