Rabbit IgG F(ab)2 Antibody Texas Red(TM) Conjugated, Goat, Polyclonal

Artikelnummer: ROC-611-1904
Artikelname: Rabbit IgG F(ab)2 Antibody Texas Red(TM) Conjugated, Goat, Polyclonal
Artikelnummer: ROC-611-1904
Hersteller Artikelnummer: 611-1904
Alternativnummer: ROC-611-1904
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: DOT
Spezies Reaktivität: Rabbit
Immunogen: Rabbit IgG F(ab)2 fragment
Konjugation: Texas Red
Alternative Synonym: Goat Anti-Rabbit IgG F(ab)2 Antibody Texas Red(TM) Conjugation, Goat Anti-Rabbit IgG Fab2 Texas Red(TM) Conjugated Antibody, Goat Anti-Rabbit IgG Fab2 Fragment Texas Red(TM) Conjugated Antibody
Klonalität: Polyclonal
Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Rabbit
Antibody Type: Secondary Antibody
Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
Anwendungsbeschreibung: Anti-Rabbit IgG F(ab)2 Texas Red Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, inclu
Western Blot of Anti-Rabbit IgG F(ab)2 (GOAT) Antibody (p/n 611-1104). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(ab)2 (GOAT) Antibody (p/n 611-1104) 1:10,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.