Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed, ALP, Guinea pig, Polyclonal

Produktübersicht

• Artikelname: Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed, ALP, Guinea pig, Polyclonal
• Artikelnummer: ROC-611-205-122
• Hersteller Artikelnummer: 611-205-122
• Alternativnummer: ROC-611-205-122
• Hersteller: Rockland Immunochemicals
• Wirt: Guinea pig
• Kategorie: Antikörper
• Spezies Reaktivität: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Konjugation: ALP
• Alternative Synonym: Guinea Pig Anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody, Guinea Pig Anti Rabbit IgG Antibody Alkaline Phosphatase Conjugation

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Rabbit
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this prod

Bilder

Western Blot of Anti-Rabbit IgG (H&L) (GUINEA PIG) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n 611-201-122). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GUINEA PIG) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n 611-201-122) 1:500 for 60 min at RT. Secondary antibody: Anti-Guinea Pig IgG (GOAT) Peroxidase Conjugated Antibody (p/n 606-103-129) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab). Rabbit F(c) migrates slightly higher.