Rabbit IgG (H&L) Antibody Pre-Adsorbed Texas Red(TM) Conjugated, Guinea pig, Polyclonal

Artikelnummer: ROC-611-209-122
Artikelname: Rabbit IgG (H&L) Antibody Pre-Adsorbed Texas Red(TM) Conjugated, Guinea pig, Polyclonal
Artikelnummer: ROC-611-209-122
Hersteller Artikelnummer: 611-209-122
Alternativnummer: ROC-611-209-122
Hersteller: Rockland Immunochemicals
Wirt: Guinea pig
Kategorie: Antikörper
Spezies Reaktivität: Rabbit
Immunogen: Rabbit IgG whole molecule
Konjugation: Texas Red
Alternative Synonym: Guinea Pig Anti-Rabbit IgG Texas Red(TM) Conjugated Antibody, Guinea Pig Anti Rabbit IgG Antibody Texas Red(TM) Conjugation
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Rabbit
Antibody Type: Secondary Antibody
Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
Anwendungsbeschreibung: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor
Western Blot of Anti-Rabbit IgG (H&L) (GUINEA PIG) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n 611-201-122). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GUINEA PIG) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n 611-201-122) 1:500 for 60 min at RT. Secondary antibody: Anti-Guinea Pig IgG (GOAT) Peroxidase Conjugated Antibody (p/n 606-103-129) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab). Rabbit F(c) migrates slightly higher.