Chicken IgG anti-Rabbit IgG (H+L)-Biotin, MinX none, Gallus, Polyclonal

Produktübersicht

• Artikelname: Chicken IgG anti-Rabbit IgG (H+L)-Biotin, MinX none, Gallus, Polyclonal
• Artikelnummer: ROC-611-906-002
• Hersteller Artikelnummer: 611-906-002
• Alternativnummer: ROC-611-906-002
• Hersteller: Rockland Immunochemicals
• Wirt: Gallus
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Konjugation: Biotin
• Alternative Synonym: Chicken Anti-Rabbit IgG Antibody Biotin Conjugated, Chicken Anti Rabbit IgG Biotin Conjugated Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rabbit
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Rabbit IgG (H&L) Biotin Antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sul

Bilder

Western Blot of Biotin Conjugated Chicken Anti-Rabbit IgG Secondary Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Rabbit IgG Antibody biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.

Western Blot of Anti-Rabbit IgG (H&L) (CHICKEN) Antibody (p/n 611-901-002). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (CHICKEN) Antibody (p/n 611-901-002) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Chicken IgG (GOAT) Peroxidase Conjugated Antibody (p/n 603-103-126) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.