Goat IgG anti-Rat IgG (Fc)-unconj., MinX none

Artikelnummer: ROC-612-1103
Artikelname: Goat IgG anti-Rat IgG (Fc)-unconj., MinX none
Artikelnummer: ROC-612-1103
Hersteller Artikelnummer: 612-1103
Alternativnummer: ROC-612-1103
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Rat
Immunogen: Rat IgG F(c) fragment
Konjugation: Unconjugated
Alternative Synonym: Goat Anti Rat IgG F(c) Antibody, Goat Anti-Rat IgG Fc Fragment Antibody, Goat Anti-Rat IgG Fc Antibody
Format: IgG
Spezifität: IgG (Fc)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 2.18 mg/ml by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Rat
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:8,000 - 1:9,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Anwendungsbeschreibung: Anti-Rat IgG F(c) antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Western blot of Goat Anti-Rat IgG F(c) secondary antibody. Lane 1: Rat Fc (p/n 012-0103). Lane 2: None. Load: 50 ng per lane. Primary antibody: Rat IgG F(c) antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Rat IgG F(c). Other band(s): None.