Anti-Rat IgG F(c) antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Western blot of Goat Anti-Rat IgG F(c) secondary antibody. Lane 1: Rat Fc (p/n 012-0103). Lane 2: None. Load: 50 ng per lane. Primary antibody: Rat IgG F(c) antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Rat IgG F(c). Other band(s): None.
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