Rat IgG (H&L) Antibody Dylight(TM) 649 Conjugated, Goat, Polyclonal

Artikelname:	Rat IgG (H&L) Antibody Dylight(TM) 649 Conjugated, Goat, Polyclonal
Artikelnummer:	ROC-612-143-002
Hersteller Artikelnummer:	612-143-002
Alternativnummer:	ROC-612-143-002
Hersteller:	Rockland Immunochemicals
Wirt:	Goat
Kategorie:	Antikörper
Applikation:	WB
Spezies Reaktivität:	Rat
Immunogen:	Rat IgG, whole molecule
Konjugation:	DyLight 649
Alternative Synonym:	Goat Anti-Rat IgG DyLight 649(TM) Conjugated Antibody, Goat Anti-Rat IgG Antibody DyLight 649(TM) Conjugation

Klonalität:	Polyclonal
Konzentration:	1.0 mg/mL by UV absorbance at 280 nm
Puffer:	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:	Lyophilized
Target-Kategorie:	Rat
Antibody Type:	Secondary Antibody

Application Verdünnung:	FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000
Anwendungsbeschreibung:	Anti-Rat IgG DyLight(TM) 649 has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this DyLight(TM) conjugate match the principle ou

	Properties of DyLight(TM) Fluorescent Dyes.

	Analysis of somatostatin (SOM)-, calretinin (CR)-, and calbindin (CB)-expressing interneurons in the cingulate cortex of FVB-Tg(GadGFP)45704Swn/J mice. A-F: Representative confocal images of GFP 1 cells (green) immunopositive for SOM (red), CR (white), and CB (blue) in layers II-III of the cingulate cortex. Open yellow arrows indicate GFP1/SOM1/CR1/CB 1 cells, solid yellow arrows GFP1/SOM1/CR 1 cells, open yellow arrowheads GFP1/SOM2/CR 1 cells, and solid yellow arrowheads GFP1/ SOM 1 cells. G: Mean 6 standard deviation of relative numbers of GFP1/SOM2/CR2/CB2 cells (a), GFP1/SOM2/CR1/CB2 cells (b), GFP1/SOM1/CR2/CB2 cells (c), GFP1/SOM1/ CR2/CB 1 cells (d), GFP1/SOM1/CR1/CB2cells (e), and GFP1/SOM1/CR1/CB 1 cells (f) in the cingulate cortex. Scale bar 5 20 lm in F (applies to A-F). Figure 7. PMID: 26669716.
	Analysis of neuropeptide Y (NPY) expressing interneurons in the cingulate cortex of FVB-Tg(GadGFP)45704Swn/J mice. A-D: Representative confocal images of cells immunopositive for GFP (green), somatostatin (SOM, white), and NPY (red), and merged image of all channels. Solid yellow arrows indicate GFP1/SOM1/NPY 1 cells and open yellow arrows GFP1/ SOM1/NPY2 cells. E: Mean 6 standard deviation of relative numbers of GFP1/SOM2/NPY2 cells, GFP1/SOM1/NPY2 cells, and GFP1/SOM1/NPY 1 cells in the cingulate cortex. Scale bar 5 20 lm in D (applies to A-D). Figure 10. PMID: 26669716.
	Analysis of somatostatin-expressing interneurons in the cingulate cortex of FVB-Tg(GadGFP)45704Swn/J mice. A,B: Representative confocal images of cells immunopositive for GAD65/67 (white), SOM (red), and GFP (green). Solid yellow arrows indicate GAD65/ 671/SOM 1 cells and open yellow arrows GAD65/671/SOM2 cells. C: Mean 6 standard deviation of relative numbers of GAD65/671/ SOM2 and GAD65/671/SOM 1 cells in the cingulate cortex. D-F: Representative confocal images of GFP 1 cells (green) in the cingulate cortex immunopositive for SOM (magenta). The combined expression of GFP and SOM in the same neuron is shown in the merged image (F). Open white arrows represent GFP1/SOM 1 cells and solid white arrows SOM1/GFP- cells. G-I: Overview of the laminar distribution of SOM 1 cells in the cingulate cortex shown in representative confocal images. The laminar distribution of GFP 1 cells in the cingulate cortex is shown in G. Layer-specific distribution of SOM 1 neurons is shown in H. The merged image of both channels is shown in I. Whereas GFP 1 cells were clearly confined to layers II-III, SOM 1 cells were also found in the deeper cortical layers. Roman numbers I-VI indicate cortical layers I-VI. J: Mean 6 standard deviation of relative numbers of GFP1/SOM 1 and GFP1/SOM2 cells in the cingulate cortex. K: Mean 6 standard deviation of relative numbers of GFP-/SOM 1 and GFP1/SOM 1 cells in the cingulate cortex. Scale bar in I 5 20 lm for A,B,D-F, 100 lm for G-I. Figure 4. PMID: 26669716.
	Western Blot of Goat anti-Rat IgG (H&L) Antibody DyLight(TM) 649 Conjugated. Lane 1: Rat IgG. Load: 50 ng per lane. Secondary antibody: Rat IgG (H&L) Antibody DyLight(TM) 649 Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa.
	DyLight(TM) dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 549 conjugate. Anti-TNFa was detected using a DyLight(TM) 649 conjugate. The image was captured using the Typhoon(TM) 9410 Imaging System.
	DyLight(TM) 649 Fluorescence Spectra.