Rat IgG Fc Antibody Rhodamine Conjugated, TRITC, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Rat IgG Fc Antibody Rhodamine Conjugated, TRITC, Rabbit, Polyclonal
• Artikelnummer: ROC-612-4003
• Hersteller Artikelnummer: 612-4003
• Alternativnummer: ROC-612-4003
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Rat
• Immunogen: Rat IgG F(c) fragment
• Konjugation: TRITC
• Alternative Synonym: Rabbit Anti-Rat IgG F(c) rhodamine Conjugated Antibody, Rabbit Anti-Rat IgG Fc Fragment rhodamine Conjugated Antibody, Rabbit Anti-Rat IgG Fc Antibody TRITC Conjugation

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.5 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rat
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: Anti-Rat IgG F(c) rhodamine conjugated antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analy

Bilder

Western Blot of Anti-Rat IgG F(c) (RABBIT) Antibody (p/n 612-4103). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG F(c) (RABBIT) Antibody (p/n 612-4103) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.