Rat IgM (mu chain) Antibody Rhodamine Conjugated, TRITC, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Rat IgM (mu chain) Antibody Rhodamine Conjugated, TRITC, Rabbit, Polyclonal
• Artikelnummer: ROC-612-4007
• Hersteller Artikelnummer: 612-4007
• Alternativnummer: ROC-612-4007
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Spezies Reaktivität: Rat
• Immunogen: Rat IgM whole molecule
• Konjugation: TRITC
• Alternative Synonym: Rabbit Anti-Rat IgM (mu chain) Antibody rhodamine Conjugated, Rabbit Anti-Rat IgM mu Antibody TRITC Conjugation

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.5 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rat
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor

Bilder

Western Blot of Anti-Rat IgM (mu chain) (RABBIT) Antibody (p/n 612-4107). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody (p/n 612-4107) 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.