Anti-NMDA NR2B Subunit pY1472 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1472.
Anti-NMDA NR2B Subunit pY1472 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 180 kDa in size corresponding to NMDAR NR2B subunit protein phos
Western Blot of Rabbit anti-NMDA NR2B Subunit Tyr1472 antibody. Lane 1: rat hippocampal lysate (Control). Lane 2: phos-pep. Load: 10 µg per lane. Primary antibody: NMDA NR2B Subunit Tyr1472 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 180 kDa for NMDA NR2B Subunit Tyr1472. Other band(s): NMDA NR2B Subunit Tyr1472 splice variants and isoforms.
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