Anti-Connexin 43 Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH.
Konjugation:
Unconjugated
Alternative Synonym:
Gap junction alpha-1 protein, Gap junction 43 kDa heart protein, CX43, Connexin-43
Klonalität:
Polyclonal
Konzentration:
titrated reagent Sufficient to run approximately 10 miniblots
Anti-Connexin 43 Antibody has been tested by western blot and is suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 43 kDa in size corresponding to connexin 43 protein in the
Western Blot of Anti-Connexin 43 (Rabbit) Antibody. Lane 1: rat cerebellar lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Connexin 43 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~43kDa/~43kDa for connexin43 protein. Other band(s): none.
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