DARPP-32 phospho S137 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: DARPP-32 phospho S137 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-612-401-D26
• Hersteller Artikelnummer: 612-401-D26
• Alternativnummer: ROC-612-401-D26
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Mouse, Rat
• Immunogen: Anti-DARPP-32 pS137 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser137 conjugated to KLH.
• Konjugation: Unconjugated
• Alternative Synonym: Protein phosphatase 1 regulatory subunit 1B, Dopamine- and cAMP-regulated neuronal phosphoprotein

Produkteigenschaften

• Klonalität: Polyclonal
• NCBI: 360616
• UniProt: Q6J4I0
• Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Formulierung: Liquid
• Target-Kategorie: Rat
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000, WB: 1:1000
• Anwendungsbeschreibung: Anti-DARPP-32 pS137 Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 32 kDa in size corresponding to DARPP 32 protein phosphorylated at Ser137

Bilder

Western Blot of Anti-DARPP-32 pS137 (Rabbit) Antibody. Lane 1: rat caudate lysate. Lane 2: rat caudate lysate blocked by preadsorption of the phospho-peptide. Load: 10 µg per lane. Primary antibody: DARPP-32 pS137 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~32kDa/~37kDa for DARPP-32 phosphorylated at Thr137. Other band(s): none.