Anti-Synaptotagmin 1 Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues specific to synaptotagmin 1.
Anti-Synaptotagmin 1 antibody is tested for use in Western Blotting. Expect a band of approximately 65 kDa in size corresponding to synaptotagmin I protein in the appropriate cell lysate or extract. Researchers should determine optimal titers for applica
Western Blot of Rabbit anti-Synaptotagmin 1 antibody. Lane 1: rat cortex lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Synaptotagmin 1 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 65 kDa for Synaptotagmin 1. Other band(s): none.
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