Rabbit IgG anti-Rat IgG (F(ab)2)-FITC, MinX none

Produktübersicht

• Artikelname: Rabbit IgG anti-Rat IgG (F(ab)2)-FITC, MinX none
• Artikelnummer: ROC-612-4204
• Hersteller Artikelnummer: 612-4204
• Alternativnummer: ROC-612-4204
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FLISA,FACS,IF
• Spezies Reaktivität: Rat
• Immunogen: Rat IgG F(ab)2 fragment
• Konjugation: FITC
• Alternative Synonym: Rabbit Anti-Rat IgG F(ab)2 fluorescein Conjugated Antibody, Rabbit Anti-Rat IgG Fab2 fluorescein Conjugated Antibody, Rabbit Anti-Rat IgG Fab2 Fragment Antibody FITC Conjugation
• Format: IgG
• Spezifität: IgG (F(ab')2)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rat
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor

Bilder

Western Blot of Anti-Rat IgG F(ab)2 (RABBIT) Antibody (p/n 612-4104). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG F(ab)2 (RABBIT) Antibody (p/n 612-4104) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.