Rabbit IgG anti-Rat IgG (F(ab)2)-Biotin, MinX none

Produktübersicht

• Artikelname: Rabbit IgG anti-Rat IgG (F(ab)2)-Biotin, MinX none
• Artikelnummer: ROC-612-4604
• Hersteller Artikelnummer: 612-4604
• Alternativnummer: ROC-612-4604
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Rat
• Immunogen: Rat IgG F(ab)2 fragment
• Konjugation: Biotin
• Alternative Synonym: Rabbit Anti Rat IgG F(ab)2 Biotin Conjugated Antibody, Rabbit Anti-Rat IgG Fab2 Biotin Conjugated Antibody, Rabbit Anti-Rat IgG Fab2 Fragment Antibody Biotin Conjugation
• Format: IgG
• Spezifität: IgG (F(ab')2)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rat
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Rat IgG F(ab)2 antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Bilder

Western Blot of Anti-Rat IgG F(ab)2 (RABBIT) Antibody (p/n 612-4104). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG F(ab)2 (RABBIT) Antibody (p/n 612-4104) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.