Rabbit IgG anti-Sheep IgG (H+L)-HRPO, MinX Hu, Polyclonal

Artikelnummer: ROC-613-4328
Artikelname: Rabbit IgG anti-Sheep IgG (H+L)-HRPO, MinX Hu, Polyclonal
Artikelnummer: ROC-613-4328
Hersteller Artikelnummer: 613-4328
Alternativnummer: ROC-613-4328
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Sheep
Immunogen: Sheep IgG whole molecule
Konjugation: HRPO
Alternative Synonym: Rabbit Anti-Sheep IgG peroxidase Conjugated Antibody, Rabbit Anti-Sheep IgG Antibody HRP Conjugation
Format: IgG
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): Human
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Sheep
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:5,000 - 1:22,000, IHC: 1:300 - 1:1,500, WB: 1:500 - 1:3,000
Anwendungsbeschreibung: Anti-Sheep IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic ass
ELISA Results of Rabbit Anti-Sheep IgG Antibody Peroxidase Conjugate (Min X Human Serum Proteins) tested against purified Sheep IgG Min X Hu HRP. Each well was coated in duplicate with 1.0 µg of Sheep IgG (p/n 013-0102). The working dilution is 1:57000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.