Donkey IgG anti-Sheep IgG (H+L)-HRPO, MinX none

Produktübersicht

• Artikelname: Donkey IgG anti-Sheep IgG (H+L)-HRPO, MinX none
• Artikelnummer: ROC-613-7302
• Hersteller Artikelnummer: 613-7302
• Alternativnummer: ROC-613-7302
• Hersteller: Rockland Immunochemicals
• Wirt: Donkey
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Sheep
• Immunogen: Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.
• Konjugation: HRPO
• Alternative Synonym: Donkey Anti-Sheep IgG peroxidase Conjugated Antibody, Donkey Anti-Sheep IgG Antibody HRP Conjugation
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Sheep
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:50,000 - 1:150,000, IHC: 1:1,000 - 1:5,000, WB: 1:3,000 - 1:15,000
• Anwendungsbeschreibung: Anti-Sheep IgG Peroxidase conjugated antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection s

Bilder

ELISA results of purified Donkey anti-Sheep IgG Antibody Peroxidase Conjugated tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 µg of Sheep IgG (p/n 003-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n TMBE-1000.